A new enzyme "Bilirubin oxidase" produced by Myrothecium verrucaria MT-1.
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چکیده
منابع مشابه
Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase.
Type III Cu ligand, His456 and His458, of Myrothecium verrucaria (MT-1) bilirubin oxidases (BO) [EC 1.3.3.5] were doubly mutated as to Lys, Asp, and Val. In spite of perturbation of the type III Cu centers, these mutants were pale blue or colourless when isolated. However, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(II), and octacyan...
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Cellulolytic enzyme preparations active against various forms of cellulose have been reported by a number of investigators who have used a variety of different sources (8). Fungi which have been employed for the preparation of such extracts are Sporotrichum cards (15), Aspergillus oryxae (3,2), Merulius lachrymans (9), and Coniophora cerebella (7), while bacteria have included Bacterium protoxo...
متن کاملPurification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190.
Myrothecium verrucaria 3.2190 is a nonligninolytic fungus that produces bilirubin oxidase. Both M. verrucaria and the extracellular bilirubin oxidase were tested for their ability to decolorize indigo carmine. The biosorption and biodegradation of the dye were detected during the process of decolorization; more than 98% decolorization efficiency was achieved after 7 days at 26°C. Additionally, ...
متن کاملSurface characterization and direct electrochemistry of redox copper centers of bilirubin oxidase from fungi Myrothecium verrucaria.
The key characteristics of multicopper oxidases are redox potentials of Type 1, Type 2 and Type 3 copper centers of enzymes. However, there is still a challenge to obtain a value of the redox "signature" of the enzymes. In this study, the electrochemical behavior of T1 and T2/T3 redox copper centers of bilirubin oxidase (BOD) from the fungi Myrothecium verrucaria was studied based on direct bio...
متن کاملEnzyme-Linked Immunosorbent Assay Detection of Trichothecenes Produced by the Bioherbicide Myrothecium verrucaria in Cell Cultures, Extracts, and Plant Tissues
A rapid technique for trichothecene detection was needed in screening tests of the potential bioherbicide Myrothecium verrucaria (MV), in order to select strains, mutants, or formulations that were void of or that possessed low amounts of these undesirable mycotoxin compounds. Commercially available enzymelinked immunosorbent assay (ELISA) plates for trichothecene detection, possessing cross-re...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1981
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.45.2383